Mouse MIP1a(Macrophage Inflammatory Protein 1 Alpha) ELISA Kit

Mouse MIP1a(Macrophage Inflammatory Protein 1 Alpha) ELISA Kit

262.00364.00

Specification 96T, 48T

SKU: ELK1116 Category: Tag:

Overview of Mouse MIP1a(Macrophage Inflammatory Protein 1 Alpha) ELISA Kit

Product name: Mouse MIP1a(Macrophage Inflammatory Protein 1 Alpha) ELISA Kit
Reactivity: Mouse
Alternative Names: MIP1 Alpha; CCL3; MIP1-A; SCYA3; Chemokine C-C-Motif Ligand 3; Small Inducible Cytokine A3; Homologous To Mouse Mip-1a; Tonsillar Lymphocyte LD78 Alpha Protein
Assay Type: Sandwich
Sensitivity: 5.9 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5 h
Research Area: Cytokine;Infection immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse MIP1a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MIP1a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse MIP1a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse MIP1a in the samples is then determined by comparing the OD of the samples to the standard curve.

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Additional information

Size

48T, 96T, 96T,48T

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