Mouse NT-ProANP-N-Terminal Pro-Atrial Natriuretic Peptide ELISA Kit
Mouse NT-ProANP-N-Terminal Pro-Atrial Natriuretic Peptide ELISA Kit
Specification 96T, 48T
Overview of Mouse NT-ProANP-N-Terminal Pro-Atrial Natriuretic Peptide ELISA Kit
| Product name: | Mouse NT-ProANP-N-Terminal Pro-Atrial Natriuretic Peptide ELISA Kit |
| Reactivity: | Mouse |
| Alternative Names: | NT-Pro-ANP; N-ANPNT-Pro-ANP; N-ANP |
| Assay Type: | Sandwich |
| Sensitivity: | 29 pg/mL |
| Standard: | 5000 ng/mL |
| Detection Range: | 78.13-5000 pg/mL |
| Sample Type: | Serum, plasma and other biological fluids. |
| Assay Length: | 3.5 h |
| Research Area: | Endocrinology;Cardiovascular biology; |
| Test Principle: | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NT-ProANP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NT-ProANP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse NT-ProANP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NT-ProANP in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Additional information
| Size | 96T,48T |
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