Name: Mouse OPG-Osteoprotegerin ELISA Kit - Gencyst
SKU: ELK1177
Availability: InStock

Mouse OPG-Osteoprotegerin ELISA Kit

€262.00 – €374.00

Specification 96T, 48T

SKU: ELK1177 Category: ELISA Kit Tag: ELISA Kit

Overview of Mouse OPG-Osteoprotegerin ELISA Kit

Product name:	Mouse OPG-Osteoprotegerin ELISA Kit
Reactivity:	Mouse
Alternative Names:	TNFRSF11B; TNFRSF11-B; OCIF; OPG; TR1; Tumor Necrosis Factor Receptor Superfamily Member 11b; Osteoclastogenesis Inhibitory Factor
Assay Type:	Sandwich
Sensitivity:	0.054 ng/mL
Standard:	10 pg/mL
Detection Range:	0.16-10 ng/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5 h
Research Area:	Cytokine;Metabolic pathway;Bone metabolism;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse OPG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse OPG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse OPG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse OPG in the samples is then determined by comparing the OD of the samples to the standard curve.