Mouse TSP-1-Thrombospondin-1 ELISA Kit
Overview of Mouse TSP-1-Thrombospondin-1 ELISA Kit
Product name: |
Mouse TSP-1-Thrombospondin-1 ELISA Kit |
Reactivity: | Mouse |
Alternative Names: | TSP1; Thrombospondin-1p180; THBS1; Thrombospondin 1 |
Assay Type: | Sandwitch |
Sensitivity: | 0.266 ng/mL |
Standard: | 40 ng/mL |
Detection Range: | 0.63-40 ng/mL |
Sample Type: | plasma, tissue homogenates and other biological fluids |
Assay Length: | 3.5 h |
Research Area: | Infection immunity;Hematology; |
Test Principle: | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse TSP-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TSP-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse TSP-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TSP-1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Additional information
Size | 48T, 96T |
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