Rabbit EDN1-Endothelin 1 ELISA Kit
Overview of Rabbit ET-1-Endothelin1 ELISA Kit
Product name: | Rabbit ET-1-Endothelin1 ELISA Kit |
Reactivity: | Rabbit |
Alternative Names: | ET1; PPET1; Preproendothelin-1; Big endothelin-1; EDN1 |
Assay Type: | Sandwich |
Sensitivity: | 2.74 pg/mL |
Standard: | 500 pg/mL |
Detection Range: | 7.82-500 pg/mL |
Sample Type: | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
Assay Length: | 3.5 h |
Research Area: | Endocrinology;Cardiovascular biology; |
Test Principle: | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit ET-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit ET-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit ET-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit ET-1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Additional information
Size | 48T, 96T, 96T,48T |
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