Rabbit IGF2-Insulin Like Growth Factor 2 ELISA Kit

Rabbit IGF2-Insulin Like Growth Factor 2 ELISA Kit

318.00455.00

Specification 96T, 48T

SKU: ELK2441 Category: Tag:

Overview of Rabbit IGF2-Insulin Like Growth Factor 2 ELISA Kit

Product name: Rabbit IGF2-Insulin Like Growth Factor 2 ELISA Kit
Reactivity: Rabbit
Alternative Names: INSIGF; IGF-II; Somatomedin A; Preptin; Insulin-like growth factor II Ala-25 Del
Assay Type: Sandwich
Sensitivity: 1.8 ng/mL
Standard: 300 pg/mL
Detection Range: 4.69-300 ng/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 3.5 h
Research Area: Tumor immunity;Endocrinology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit IGF2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit IGF2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit IGF2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit IGF2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Read More Description

dNTP Mix PCR Grade 10 mM each

Pfu DNA Polymerase

One Step SYBR Green RT-PCR Mix

Hot start Taq DNA Polymerase

EntiLink PCR Master Mix

Additional information

Size

48T, 96T

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