Name: Rabbit TNFa-Tumor Necrosis Factor Alpha ELISA Kit - Gencyst
SKU: ELK1164
Availability: InStock

Rabbit TNFa-Tumor Necrosis Factor Alpha ELISA Kit

€262.00 – €374.00

Specification 96T, 48T

Size

Clear

SKU: ELK1164 Category: ELISA Kit Tag: ELISA Kit

Overview of Rabbit TNFa-Tumor Necrosis Factor Alpha ELISA Kit

Product name:	Rabbit TNFa-Tumor Necrosis Factor Alpha ELISA Kit
Reactivity:	Rabbit
Alternative Names:	TNF Alpha; DIF; TNF-A; TNFSF2; Cachectin; Tumor Necrosis Factor Ligand Superfamily Member 2
Assay Type:	Sandwich
Sensitivity:	1.17 pg/mL
Standard:	200 pg/mL
Detection Range:	3.13-200 pg/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5 h
Research Area:	Cytokine;Tumor immunity;Infection immunity;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit TNFa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit TNFa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit TNFa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit TNFa in the samples is then determined by comparing the OD of the samples to the standard curve.