Rat ADH1-Alcohol Dehydrogenase 1 ELISA Kit
Rat ADH1-Alcohol Dehydrogenase 1 ELISA Kit
€318.00 – €455.00
Specification 96T, 48T
Overview of Rat ADH1-Alcohol Dehydrogenase 1 ELISA Kit
| Product name: | Rat ADH1-Alcohol Dehydrogenase 1 ELISA Kit |
| Reactivity: | Rat |
| Alternative Names: | ADH1A; Alcohol Dehydrogenase 1A(Class I)Alpha Polypeptide; Alcohol dehydrogenase subunit alpha |
| Assay Type: | Sandwich |
| Sensitivity: | 0.122 ng/mL |
| Standard: | 20 ng/mL |
| Detection Range: | 0.32-20 ng/mL |
| Sample Type: | Serum, plasma, tissue homogenates and other biological fluids |
| Assay Length: | 3.5 h |
| Research Area: | Enzyme & Kinase;Metabolic pathway;Hepatology; |
| Test Principle: | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ADH1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ADH1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ADH1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ADH1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
Read More Description
Additional information
| Size | 48T, 96T |
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