Rat bCTx-Beta-Crosslaps ELISA Kit
Overview of Rat bCTx-Beta-Crosslaps ELISA Kit
Product name: |
Rat bCTx-Beta-Crosslaps ELISA Kit |
Reactivity: | Rat |
Alternative Names: | Beta CTx; b-CTx; bCTXI; bCTX-I; B-Cr; BCL; Type I Collagen C-Telopeptide-Related Fraction |
Assay Type: | Competitive Inhibition |
Sensitivity: | 37.5 pg/mL |
Standard: | 8000 ng/mL |
Detection Range: | 125-8000 pg/mL |
Sample Type: | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
Assay Length: | 2 h |
Research Area: | Bone metabolism; |
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat bCTx protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat bCTx. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat bCTx in the samples is then determined by comparing the OD of the samples to the standard curve. |
Read More Description
Additional information
Size | 48T, 96T |
---|
Reviews
There are no reviews yet.