Rat COL3-Collagen Type III ELISA Kit
Rat COL3-Collagen Type III ELISA Kit
Specification 96T, 48T
Overview of Rat COL3-Collagen Type III ELISA Kit
| Product name: | Rat COL3-Collagen Type III ELISA Kit |
| Reactivity: | Rat |
| Alternative Names: | Collagen Type 3; Col 3; Collagen Type III; Col III |
| Assay Type: | Sandwich |
| Sensitivity: | 0.122 ng/mL |
| Standard: | 20 pg/mL |
| Detection Range: | 0.32-20 ng/mL |
| Sample Type: | Serum, plasma, tissue homogenates and other biological fluids |
| Assay Length: | 3.5 h |
| Research Area: | Metabolic pathway;Cardiovascular biology; |
| Test Principle: | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat COL3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat COL3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat COL3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat COL3 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Additional information
| Size | 96T,48T |
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