Name: Rat DAO-Diamine Oxidase ELISA Kit - Gencyst
SKU: ELK2230
Availability: InStock

Rat DAO-Diamine Oxidase ELISA Kit

€318.00 – €455.00

Specification 96T, 48T

Size

SKU: ELK2230 Category: ELISA Kit Tag: ELISA Kit

Overview of Rat DAO-Diamine Oxidase ELISA Kit

Product name:	Rat DAO-Diamine Oxidase ELISA Kit
Reactivity:	Rat
Alternative Names:	AOC1; ABP1; KAO; Histaminase; Kidney amine oxidase; Amiloride Binding Protein 1; Amiloride-sensitive amine oxidase; Amine oxidase copper domain-containing protein 1
Assay Type:	Sandwich
Sensitivity:	0.236 ng/mL
Standard:	40 pg/mL
Detection Range:	0.63-40 ng/mL
Sample Type:	Serum, plasma, tissue homogenates, cell lysates and other biological fluids
Assay Length:	3.5 h
Research Area:	Enzyme & Kinase;
Test Principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat DAO. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat DAO. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat DAO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat DAO in the samples is then determined by comparing the OD of the samples to the standard curve.

Size	48T, 96T

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