Name: Rat EPO-Erythropoietin ELISA Kit - Gencyst
SKU: ELK2483
Availability: InStock

Rat EPO-Erythropoietin ELISA Kit

€318.00 – €455.00

Specification 96T, 48T

Size

SKU: ELK2483 Category: ELISA Kit Tag: ELISA Kit

Overview of Rat EPO-Erythropoietin ELISA Kit

Product name:	Rat EPO-Erythropoietin ELISA Kit
Reactivity:	Rat
Alternative Names:	EP; Epoetin; Erythropoetin; Hematopoietin; Hemopoietin
Assay Type:	Sandwich
Sensitivity:	35.22 pg/mL
Standard:	6000 pg/mL
Detection Range:	93.75-6000 pg/mL
Sample Type:	serum, plasma, tissue homogenates and other biological fluids
Assay Length:	3.5 h
Research Area:	Cytokine;Endocrinology;Hematology;Hormone metabolism;
Test Principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat EPO. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat EPO. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat EPO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat EPO in the samples is then determined by comparing the OD of the samples to the standard curve.

Size	48T, 96T