Rat FABP2-Fatty Acid Binding Protein 2-Intestinal-ELISA Kit

Rat FABP2-Fatty Acid Binding Protein 2-Intestinal-ELISA Kit

320.00458.00

Specification 96T, 48T

SKU: ELK2603 Category: Tag:

Overview of Rat FABP2-Fatty Acid Binding Protein 2-Intestinal-ELISA Kit

Product name: Rat FABP2-Fatty Acid Binding Protein 2-Intestinal-ELISA Kit
Reactivity: Rat
Alternative Names: FABPI; IFABP; I-FABP; Intestinal-type fatty acid-binding protein; FABP2; Fatty Acid Binding Protein 2, Intestinal
Assay Type: Sandwich
Sensitivity: 0.133 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Metabolic pathway;Tumor immunity;Endocrinology;Cardiovascular biology;
Uniprot ID: P02693
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IFABP/FABP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IFABP/FABP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat IFABP/FABP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IFABP/FABP2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Read More Description

dNTP Mix PCR Grade 10 mM each

Pfu DNA Polymerase

One Step SYBR Green RT-PCR Mix

Hot start Taq DNA Polymerase

EntiLink PCR Master Mix

Additional information

Size

48T, 96T, 96T,48T

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