Rat FGF4-Fibroblast Growth Factor 4 ELISA Kit

Rat FGF4-Fibroblast Growth Factor 4 ELISA Kit

262.00374.00

Specification 96T, 48T

SKU: ELK2490 Category: Tag:

Overview of Rat FGF4-Fibroblast Growth Factor 4 ELISA Kit

Product name: Rat FGF4-Fibroblast Growth Factor 4 ELISA Kit
Reactivity: Rat
Alternative Names: HBGF4; HST; HSTF1; KFGF; K-FGF; Heparin Secretory Transforming Protein 1; Kaposi Sarcoma Oncogene; Transforming Protein KS3; FGF-Related Oncogene
Assay Type: Sandwich
Sensitivity: 5.4 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 3.5 h
Research Area: Cytokine;Tumor immunity;Infection immunity;
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat FGF4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat FGF4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat FGF4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat FGF4 in the samples is then determined by comparing the OD of the samples to the standard curve.

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Size

48T, 96T, 96T,48T

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