Name: Rat G6PD-Glucose 6 Phosphate Dehydrogenase ELISA Kit - Gencyst
SKU: ELK2458
Availability: InStock

Rat G6PD-Glucose 6 Phosphate Dehydrogenase ELISA Kit

€318.00 – €455.00

Specification 96T, 48T

Size

Clear

SKU: ELK2458 Category: ELISA Kit Tag: ELISA Kit

Overview of Rat G6PD-Glucose 6 Phosphate Dehydrogenase ELISA Kit

Product name:	Rat G6PD-Glucose 6 Phosphate Dehydrogenase ELISA Kit
Reactivity:	Rat
Alternative Names:	Glucose-6-phosphate 1-dehydrogenase
Assay Type:	Sandwich
Sensitivity:	0.116 U/L
Standard:	20 U/L
Detection Range:	0.32-20 U/L
Sample Type:	Serum, plasma, tissue homogenates and other biological fluids.
Assay Length:	3.5 h
Research Area:	Metabolic pathway;
Test Principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat G6PD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat G6PD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat G6PD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat G6PD in the samples is then determined by comparing the OD of the samples to the standard curve.

Size	48T, 96T, 96T,48T