Rat GIP-Gastric Inhibitory Polypeptide ELISA Kit
Rat GIP-Gastric Inhibitory Polypeptide ELISA Kit
Price range: €318.00 through €455.00
Specification 96T, 48T
Overview of Rat GIP-Gastric Inhibitory Polypeptide ELISA Kit
Product name: | Rat GIP-Gastric Inhibitory Polypeptide ELISA Kit |
Reactivity: | Rat |
Alternative Names: | Incretin hormone; Glucose Dependent Insulinotropic Peptide |
Assay Type: | Competitive Inhibition |
Sensitivity: | 27.1 pg/mL |
Standard: | 5000 ng/mL |
Detection Range: | 78.13-5000 pg/mL |
Sample Type: | Serum, plasma and other biological fluids |
Assay Length: | 2 h |
Research Area: | Endocrinology;Gastroenterology;Hormone metabolism; |
Test Principle: | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat GIP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GIP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat GIP in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Additional information
Size | 48T, 96T |
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