Rat HMGB-1-High Mobility Group Protein B1 ELISA Kit
Rat HMGB-1-High Mobility Group Protein B1 ELISA Kit
Price range: €318.00 through €455.00
Specification 96T, 48T
Overview of Rat HMGB-1-High Mobility Group Protein B1 ELISA Kit
Product name: | Rat HMGB-1-High Mobility Group Protein B1 ELISA Kit |
Reactivity: | Rat |
Alternative Names: | HMGB1; HMG3; SBP1; Sulfoglucuronyl Carbohydrate Binding Protein; Amphoterin; High Mobility Group Box 1 Protein; HMG1; High Mobility Group Protein 1 |
Assay Type: | Sandwich |
Sensitivity: | 6.7 pg/mL |
Standard: | 1000 ng/mL |
Detection Range: | 15.63-1000 pg/mL |
Sample Type: | serum, plasma, tissue homogenates and other biological fluids |
Assay Length: | 3.5 h |
Research Area: | Infection immunity; |
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat HMGB-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat HMGB-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat HMGB-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat HMGB-1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Additional information
Size | 48T, 96T |
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