Name: Rat IGF1-Insulin Like Growth Factor 1 ELISA Kit - Gencyst
SKU: ELK1136
Availability: InStock

Rat IGF1-Insulin Like Growth Factor 1 ELISA Kit

€262.00 – €364.00

Specification 96T, 48T

Size

SKU: ELK1136 Category: ELISA Kit Tag: ELISA Kit

Overview of Rat IGF1-Insulin Like Growth Factor 1 ELISA Kit

Product name:	Rat IGF1-Insulin Like Growth Factor 1 ELISA Kit
Reactivity:	Rat
Alternative Names:	IGFI; IGF1A; IBP1; MGF; Somatomedin C; Mechano Growth Gactor
Assay Type:	Sandwich
Sensitivity:	0.069 ng/mL
Standard:	10 pg/mL
Detection Range:	0.16-10 ng/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5 h
Research Area:	Tumor immunity;Endocrinology;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IGF1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IGF1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat IGF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IGF1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Size	48T, 96T

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