Rat IgG(Immunoglobulin G) ELISA Kit

Rat IgG(Immunoglobulin G) ELISA Kit


Specification 96T, 48T

SKU: ELK1254 Category: Tag:

Overview of Rat IgG(Immunoglobulin G) ELISA Kit

Product name: Rat IgG(Immunoglobulin G) ELISA Kit
Reactivity: Rat
Alternative Names: Immunoglobulin G
Assay Type: Competitive Inhibition
Sensitivity: 0.49 μg/mL
Standard: 100 μg/mL
Detection Range: 1.57-100 μg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 2 h
Research Area: Infection immunity;Immune molecule;Hematology;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat IgG protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IgG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After the TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IgG in the samples is then determined by comparing the OD of the samples to the standard curve.


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48T, 96T, 96T,48T