Name: Rat IL-12p40-Interleukin 12 p40 ELISA Kit - Gencyst
SKU: ELK2509
Availability: InStock

Rat IL-12p40-Interleukin 12 p40 ELISA Kit

Price range: €318.00 through €455.00

Specification 96T, 48T

Size

Clear

SKU: ELK2509 Category: ELISA Kit Tag: ELISA Kit

Overview of Rat IL-12p40-Interleukin 12 p40 ELISA Kit

Product name:
Reactivity:	RatRat IL-12p40-Interleukin 12 p40 ELISA Kit
Alternative Names:	IL-12B; p40; IL12B; CLMF2; NKSF2; Natural Killer Cell Stimulatory Factor 2; Cytotoxic Lymphocyte Maturation Factor 2; Interleukin 23, P40 Subunit
Assay Type:	Sandwich
Sensitivity:	5.8 pg/mL
Standard:	1000 pg/mL
Detection Range:	15.63-1000 pg/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5 h
Research Area:	Cytokine;Infection immunity;Immune molecule;
	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IL-12p40. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IL-12p40. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat IL-12p40, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IL-12p40 in the samples is then determined by comparing the OD of the samples to the standard curve.