Rat IL1a(Interleukin 1 Alpha) ELISA Kit

Rat IL1a(Interleukin 1 Alpha) ELISA Kit

262.00364.00

Specification 96T, 48T

SKU: ELK1148 Category: Tag:

Overview of Rat IL1a(Interleukin 1 Alpha) ELISA Kit

Product name: Rat IL1a(Interleukin 1 Alpha) ELISA Kit
Reactivity: Rat
Alternative Names: IL1 Alpha; IL1-A; IL-1α;IL-1a; IL1F1; Preinterleukin 1 Alpha; Hematopoietin-1; Pro-Interleukin-1-Alpha; Interleukin-1 Family Member 1
Assay Type: Sandwich
Sensitivity: 6.1 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Cytokine;Apoptosis;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IL1a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IL1a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After the TMB substrate solution is added, only those wells that contain Rat IL1a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a colour change. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IL1a in the samples is then determined by comparing the OD of the samples to the standard curve.

 

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Additional information

Size

48T, 96T, 96T,48T

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