Name: Rat IL5-Interleukin 5 ELISA Kit - Gencyst
SKU: ELK2552
Availability: InStock

Rat IL5-Interleukin 5 ELISA Kit

€318.00 – €455.00

Specification 96T, 48T

Size

SKU: ELK2552 Category: ELISA Kit Tag: ELISA Kit

Overview of Rat IL5-Interleukin 5 ELISA Kit

Product name:	Rat IL5-Interleukin 5 ELISA Kit
Reactivity:	Rat
Alternative Names:	IL-5;E-CSF; ESGF; EDF; TRF; Colony-Stimulating Factor,Eosinophil; T-Cell replacing Factor; B Cell Differentiation Factor I; Eosinophil Differentiation Factor
Assay Type:	Sandwich
Sensitivity:	3.1 pg/mL
Standard:	500 pg/mL
Detection Range:	7.82-500 pg/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5 h
Research Area:	Cytokine;Infection immunity;
	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IL5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IL5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat IL5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IL5 in the samples is then determined by comparing the OD of the samples to the standard curve.

Size	48T, 96T