Rat NT-ProANP-N-Terminal Pro-Atrial Natriuretic Peptide ELISA Kit
Rat NT-ProANP-N-Terminal Pro-Atrial Natriuretic Peptide ELISA Kit
Price range: €318.00 through €455.00
Specification 96T, 48T
Overview of Rat NT-ProANP-N-Terminal Pro-Atrial Natriuretic Peptide ELISA Kit
| Product name: | Rat NT-ProANP-N-Terminal Pro-Atrial Natriuretic Peptide ELISA Kit | 
| Reactivity: | Rat | 
| Alternative Names: | NT-Pro-ANP; N-ANP | 
| Assay Type: | Sandwich | 
| Sensitivity: | 0.247 ng/mL | 
| Standard: | 40 pg/mL | 
| Detection Range: | 0.63-40 ng/mL | 
| Sample Type: | Serum, plasma, tissue homogenates and other biological fluids. | 
| Assay Length: | 3.5 h | 
| Research Area: | Endocrinology;Cardiovascular biology; | 
| Test Principle: | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NT-ProANP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NT-ProANP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat NT-ProANP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NT-ProANP in the samples is then determined by comparing the OD of the samples to the standard curve. | 
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Additional information
| Size | 48T, 96T  | 
		
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