Overview of Rat NT-ProANP-N-Terminal Pro-Atrial Natriuretic Peptide ELISA Kit
| Product name: |
Rat NT-ProANP-N-Terminal Pro-Atrial Natriuretic Peptide ELISA Kit |
| Reactivity: |
Rat |
| Alternative Names: |
NT-Pro-ANP; N-ANP |
| Assay Type: |
Sandwich |
| Sensitivity: |
0.247 ng/mL |
| Standard: |
40 pg/mL |
| Detection Range: |
0.63-40 ng/mL |
| Sample Type: |
Serum, plasma, tissue homogenates and other biological fluids. |
| Assay Length: |
3.5 h |
| Research Area: |
Endocrinology;Cardiovascular biology; |
| Test Principle: |
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NT-ProANP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NT-ProANP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat NT-ProANP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NT-ProANP in the samples is then determined by comparing the OD of the samples to the standard curve. |
dNTP Mix PCR Grade 10 mM each
Pfu DNA Polymerase
One Step SYBR Green RT-PCR Mix
Hot start Taq DNA Polymerase
EntiLink PCR Master Mix
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