Name: Rat PF4-Platelet Factor 4 ELISA Kit - Gencyst
SKU: ELK2446
Availability: InStock

Rat PF4-Platelet Factor 4 ELISA Kit

€318.00 – €455.00

Specification 96T, 48T

SKU: ELK2446 Category: ELISA Kit Tag: ELISA Kit

Overview of Rat PF4-Platelet Factor 4 ELISA Kit

Product name:	Rat PF4-Platelet Factor 4 ELISA Kit
Reactivity:	Rat
Alternative Names:	CXCL4; SCYB4; Chemokine C-X-C-Motif Ligand 4; Oncostatin-A; Iroplact
Assay Type:	Sandwich
Sensitivity:	0.137 ng/mL
Standard:	20 pg/mL
Detection Range:	0.32-20 ng/mL
Sample Type:	serum, platelet-poor plasma and cell culture supernates
Assay Length:	3.5 h
Research Area:	Cytokine;Hematology;
Test Principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PF4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PF4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PF4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PF4 in the samples is then determined by comparing the OD of the samples to the standard curve.

Size	48T, 96T, 96T,48T

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