Rat PYGL-Glycogen Phosphorylase, Liver ELISA Kit

Rat PYGL-Glycogen Phosphorylase, Liver ELISA Kit

318.00455.00

Specification 96T, 48T

SKU: ELK1578 Category: Tag:

Overview of Rat PYGL-Glycogen Phosphorylase, Liver ELISA Kit

Product name: Rat PYGL-Glycogen Phosphorylase, Liver ELISA Kit
Reactivity: Rat
Alternative Names: GPLL; GPBB; Hers Disease; Glycogen Storage Disease Type VI
Assay Type: Sandwich
Sensitivity: 0.56 ng/mL
Standard: 100 ng/mL
Detection Range: 1.57-100 ng/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
Assay Length: 3.5 h
Research Area: Enzyme & Kinase;Metabolic pathway;Endocrinology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PYGL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PYGL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PYGL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PYGL in the samples is then determined by comparing the OD of the samples to the standard curve.

Read More Description

dNTP Mix PCR Grade 10 mM each

Pfu DNA Polymerase

One Step SYBR Green RT-PCR Mix

Hot start Taq DNA Polymerase

EntiLink PCR Master Mix

Additional information

Size

48T, 96T

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