Rat TAP-Trypsinogen Activation Peptide ELISA Kit

Rat TAP-Trypsinogen Activation Peptide ELISA Kit

318.00455.00

Specification 96T, 48T

SKU: ELK1723 Category: Tag:

Overview of Rat TAP-Trypsinogen Activation Peptide ELISA Kit

Product name: Rat TAP-Trypsinogen Activation Peptide ELISA Kit
Reactivity: Rat
Alternative Names: Trypsinogen Activation Peptide
Assay Type: Competitive Inhibition
Sensitivity: 50.3 pg/mL
Standard: 10000 ng/mL
Detection Range: 156.25-10000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 2 h
Research Area: Infection immunity;Immune molecule;Hepatology;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat TAP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TAP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TAP in the samples is then determined by comparing the OD of the samples to the standard curve.

Read More Description

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Additional information

Size

48T, 96T

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