Human ACE(Angiotensin I Converting Enzyme) ELISA Kit

Human ACE(Angiotensin I Converting Enzyme) ELISA Kit

262.00364.00

Specification 96T, 48T

SKU: ELK1000 Category: Tag:

Overview of Human ACE(Angiotensin I Converting Enzyme) ELISA Kit

Product name: Human ACE(Angiotensin I Converting Enzyme) ELISA Kit
Reactivity: Human
Alternative Names: CD143; ACE1; DCP1; ACEI; ACE-I; Kininase II; Angiotensin-Converting Enzyme; Peptidyl-Dipeptidase A; Dipeptidyl Carboxypeptidase 1; Angiotensin-converting enzyme, soluble form
Assay Type: Sandwich
Sensitivity: 0.16 ng/mL
Standard: 25 pg/mL
Detection Range: 0.4-25 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Enzyme & Kinase;Cardiovascular biology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ACE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ACE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ACE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a colour change. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ACE in the samples is then determined by comparing the OD of the samples to the standard curve.

 

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Additional information

Size

48T, 96T, 96T,48T

Overview

Human ACE(Angiotensin I Converting Enzyme) ELISA Kit

Alternative Names (Others Name):

AR; CRDGF; SDGF; Colorectum Cell-Derived Growth Factor; Schwannoma-Derived Growth Factor

 

Type of Assay:

Sandwich

Sensitivity (Percision):

5.8 pg/mL

Standard:

1000 pg/mL

 

Range:

15.63-1000 pg/mL

Type of Sample:

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Length of Assay:

3.5h

Area of Research:

Cytokine;Tumor immunity;

Reactivity:

Mouse

Publications

 

Linearity

 

Matrix
1:2 1:4 1:8 1:16
serum(n=5) 78-92% 85-96% 93-102% 95-104%
EDTA plasma(n=5) 86-97% 91-102% 81-97% 93-107%
Heparin plasma(n=5) 82-94% 83-96% 91-103% 82-99%

 

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AREG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Recovery

 

Matrix Recovery range Average
serum(n=5) 93-101% 97%
EDTA plasma(n=5) 85-103% 94%
Heparin plasma(n=5) 95-105% 100%

 

Matrices listed below were spiked with certain level of recombinant AREG and the recovery rates were calculated by comparing the measured value to the expected amount of AREG in samples.

Precision

 

Intra-assay Precision (Precision within an assay):CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays):CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Standard curve

 

Concentration (ng/mL)
OD
Corrected OD
1000.00 2.154 2.046
500.00 1.647 1.539
250.00 1.157 1.049
125.00 0.726 0.618
62.50 0.514 0.406
31.25 0.318 0.210
15.63 0.216 0.108
0.00 0.108 0.000