Mouse GDNF-Glial Cell Line Derived Neurotrophic Factor ELISA Kit

Mouse GDNF-Glial Cell Line Derived Neurotrophic Factor ELISA Kit

318.00455.00

Specification 96T, 48T

SKU: ELK2495 Category: Tag:

Overview of Mouse GDNF-Glial Cell Line Derived Neurotrophic Factor ELISA Kit

Product name: Mouse GDNF-Glial Cell Line Derived Neurotrophic Factor ELISA Kit
Reactivity: Mouse
Alternative Names: ATF1; ATF2; HFB1-GDNF; Astrocyte-Derived Trophic Factor; Glial Derived Neurotrophic Factor
Assay Type: Sandwich
Sensitivity: 12.1 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5 h
Research Area: Cytokine;Apoptosis;Neuro science;
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse GDNF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GDNF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse GDNF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse GDNF in the samples is then determined by comparing the OD of the samples to the standard curve.

Read More Description

dNTP Mix PCR Grade 10 mM each

Pfu DNA Polymerase

One Step SYBR Green RT-PCR Mix

Hot start Taq DNA Polymerase

EntiLink PCR Master Mix

Additional information

Size

48T, 96T

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Overview

Mouse GDNF-Glial Cell Line Derived Neurotrophic Factor ELISA Kit

Alternative Names (Others Name):

AR; CRDGF; SDGF; Colorectum Cell-Derived Growth Factor; Schwannoma-Derived Growth Factor

 

Type of Assay:

Sandwich

Sensitivity (Percision):

5.8 pg/mL

Standard:

1000 pg/mL

 

Range:

15.63-1000 pg/mL

Type of Sample:

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Length of Assay:

3.5h

Area of Research:

Cytokine;Tumor immunity;

Reactivity:

Mouse

Publications

 

Linearity

 

Matrix
1:2 1:4 1:8 1:16
serum(n=5) 78-92% 85-96% 93-102% 95-104%
EDTA plasma(n=5) 86-97% 91-102% 81-97% 93-107%
Heparin plasma(n=5) 82-94% 83-96% 91-103% 82-99%

 

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AREG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Recovery

 

Matrix Recovery range Average
serum(n=5) 93-101% 97%
EDTA plasma(n=5) 85-103% 94%
Heparin plasma(n=5) 95-105% 100%

 

Matrices listed below were spiked with certain level of recombinant AREG and the recovery rates were calculated by comparing the measured value to the expected amount of AREG in samples.

Precision

 

Intra-assay Precision (Precision within an assay):CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays):CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Standard curve

 

Concentration (ng/mL)
OD
Corrected OD
1000.00 2.154 2.046
500.00 1.647 1.539
250.00 1.157 1.049
125.00 0.726 0.618
62.50 0.514 0.406
31.25 0.318 0.210
15.63 0.216 0.108
0.00 0.108 0.000

 

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