General E2-Estradiol ELISA Kit
General E2-Estradiol ELISA Kit
€262.00 – €374.00
Specification 96T, 48T
Overview of E2-Estradiol ELISA Kit
| Product name: | E2-Estradiol ELISA Kit |
| Reactivity: | General |
| Alternative Names: | 17B-Estradiol; Oestradiol; Beta-Estradiol |
| Assay Type: | Competitive Inhibition |
| Sensitivity: | 4.45 pg/mL |
| Standard: | 1000 ng/mL |
| Detection Range: | 15.63-1000 pg/mL |
| Sample Type: | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay Length: | 2 h |
| Research Area: | Endocrinology;Reproductive science;Hormone metabolism; |
| Test principle: | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with E2 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to E2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of E2 in the samples is then determined by comparing the OD of the samples to the standard curve. |
Read More Description
One Step SYBR Green RT-PCR Mix
Additional information
| Size | 48T, 96T |
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