Name: Dog IL1b-Interleukin 1 Beta ELISA Kit - Gencyst
SKU: ELK1296
Availability: InStock

Dog IL1b-Interleukin 1 Beta ELISA Kit

€262.00 – €374.00

Specification 96T, 48T

SKU: ELK1296 Category: ELISA Kit Tag: ELISA Kit

Overview of Dog IL1b-Interleukin 1 Beta ELISA Kit

Product name:	Dog IL1b-Interleukin 1 Beta ELISA Kit
Reactivity:	Dog
Alternative Names:	IL-1B;IL1-B; IL1-Beta; IL1F2; IL-1β; Interleukin-1 Family Member 2; Catabolin
Assay Type:	Sandwich
Sensitivity:	3.1 pg/mL
Standard:	500 ng/mL
Detection Range:	7.82-500 pg/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5 h
Research Area:	Cytokine;Tumor immunity;Infection immunity;
	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog IL1b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog IL1b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog IL1b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog IL1b in the samples is then determined by comparing the OD of the samples to the standard curve.

Size	48T, 96T

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