Human VEGF145-Vascular Endothelial Growth Factor 145 ELISA Kit

Human VEGF145-Vascular Endothelial Growth Factor 145 ELISA Kit

262.00364.00

Specification 96T, 48T

SKU: ELK1347 Category: Tag:

Overview of Human VEGF145-Vascular Endothelial Growth Factor 145 ELISA Kit

Product name: Human VEGF145-Vascular Endothelial Growth Factor 145 ELISA Kit
Reactivity: Human
Alternative Names: Vascular Endothelial Growth Factor 145
Assay Type: Sandwich
Sensitivity: 5.9 pg/mL
Standard: 1000 ng/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay Length: 3.5 h
Research Area: Cytokine;Apoptosis;Tumor immunity;
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human VEGF145. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human VEGF145. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human VEGF145, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VEGF145 in the samples is then determined by comparing the OD of the samples to the standard curve.

Read More Description

dNTP Mix PCR Grade 10 mM each

Pfu DNA Polymerase

One Step SYBR Green RT-PCR Mix

Hot start Taq DNA Polymerase

EntiLink PCR Master Mix

Additional information

Size

48T, 96T

Reviews

There are no reviews yet.

Be the first to review “Human VEGF145-Vascular Endothelial Growth Factor 145 ELISA Kit”

Your email address will not be published. Required fields are marked *

Filter by price