Name: Rat NOS3-Nitric Oxide Synthase 3, Endothelial ELISA Kit - Gencyst
SKU: ELK1501
Availability: InStock

Rat NOS3-Nitric Oxide Synthase 3, Endothelial ELISA Kit

€318.00 – €455.00

Specification 96T, 48T

Size

Clear

SKU: ELK1501 Category: ELISA Kit Tag: ELISA Kit

Overview of Rat NOS3-Nitric Oxide Synthase 3, Endothelial ELISA Kit

Product name:	Rat NOS3-Nitric Oxide Synthase 3, Endothelial ELISA Kit
Reactivity:	Rat
Alternative Names:	ECNOS; NOS III; E-NOS; C-NOS; ENOS; CNOS; Constitutive NOS
Assay Type:	Sandwich
Sensitivity:	0.59 ng/mL
Standard:	100 ng/mL
Detection Range:	1.57-100 ng/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates and other biological fluids
Assay Length:	3.5 h
Research Area:	Enzyme & Kinase;Cardiovascular biology;
	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NOS3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NOS3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat NOS3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NOS3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Size	48T, 96T

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