Mouse NOS2/iNOS-NitricOxide Synthase 2, Inducible ELISA Kit
Mouse NOS2/iNOS-NitricOxide Synthase 2, Inducible ELISA Kit
€318.00 – €455.00
Specification 96T, 48T
Overview of Mouse NOS2/iNOS-NitricOxide Synthase 2, Inducible ELISA Kit
| Product name: | Mouse NOS2/iNOS-NitricOxide Synthase 2, Inducible ELISA Kit |
| Reactivity: | Mouse |
| Alternative Names: | NOS2A; INOS; HEP-NOS; I-NOS; Hepatocytes Oxide Synthase; Peptidyl-cysteine S-nitrosylase NOS2; NOS2; Nitric Oxide Synthase 2, Inducible |
| Assay Type: | Sandwich |
| Sensitivity: | 0.116 ng/mL |
| Standard: | 20 ng/mL |
| Detection Range: | 0.32-20 ng/mL |
| Sample Type: | tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay Length: | 3.5 h |
| Research Area: | Enzyme & Kinase;Metabolic pathway;Tumor immunity;Cardiovascular biology; |
| Test principle: | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NOS2/iNOS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NOS2/iNOS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse NOS2/iNOS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NOS2/iNOS in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Additional information
| Size | 48T, 96T |
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