Mouse LIPD-Lipase, Lipoprotein ELISA Kit
Overview of Mouse LIPD-Lipase, Lipoprotein ELISA Kit
Product name: | Mouse LIPD-Lipase, Lipoprotein ELISA Kit |
Reactivity: | Mouse |
Alternative Names: | LPL; Lipoprotein Lipase |
Assay Type: | Sandwich |
Sensitivity: | 0.61 ng/mL |
Standard: | 100 ng/mL |
Detection Range: | 1.57-100 ng/mL |
Sample Type: | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
Assay Length: | 3.5 h |
Research Area: | Enzyme & Kinase;Metabolic pathway;Cardiovascular biology; |
Test Principle: | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse LIPD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse LIPD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse LIPD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse LIPD in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Additional information
Size | 48T, 96T |
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