Name: Rat PAR2-Protease Activated Receptor 2 ELISA Kit - Gencyst
SKU: ELK1638
Availability: InStock

Rat PAR2-Protease Activated Receptor 2 ELISA Kit

€318.00 – €455.00

Specification 96T, 48T

Size

Clear

SKU: ELK1638 Category: ELISA Kit Tag: ELISA Kit

Overview of Rat PAR2-Protease Activated Receptor 2 ELISA Kit

Product name:	Rat PAR2-Protease Activated Receptor 2 ELISA Kit
Reactivity:	Rat
Alternative Names:	F2RL1; F2-RL1; GPR11; PAR2; Coagulation Factor II(thrombin)receptor-Like 1; G-protein coupled receptor 11; Thrombin receptor-like 1
Assay Type:	Sandwich
Sensitivity:	0.053 ng/mL
Standard:	10 ng/mL
Detection Range:	0.16-10 ng/mL
Sample Type:	Tissue homogenates, cell lysates and other biological fluids
Assay Length:	3.5 h
Research Area:	Signal transduction;
	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PAR2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PAR2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PAR2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PAR2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Size	48T, 96T, 96T,48T

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