Name: Human AIF-Apoptosis Inducing Factor ELISA Kit - Gencyst
SKU: ELK1670
Availability: InStock

Human AIF-Apoptosis Inducing Factor ELISA Kit

€318.00 – €455.00

Specification 96T, 48T

Size

Clear

SKU: ELK1670 Category: ELISA Kit Tag: ELISA Kit

Overview of Human AIF-Apoptosis Inducing Factor ELISA Kit

Product name:	Human AIF-Apoptosis Inducing Factor ELISA Kit
Reactivity:	Human
Alternative Names:	PDCD8; Programmed Cell Death protein 8; Apoptosis-inducing factor 1, mitochondrial
Assay Type:	Sandwich
Sensitivity:	5.5 pg/mL
Standard:	1000 ng/mL
Detection Range:	15.63-1000 pg/mL
Sample Type:	serum, plasma, tissue homogenates and other biological fluids
Assay Length:	3.5 h
Research Area:	Signal transduction;Apoptosis;
Test Principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human AIF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AIF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human AIF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AIF in the samples is then determined by comparing the OD of the samples to the standard curve.

Size	48T, 96T

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