Name: Human PON1-Paraoxonase 1 ELISA Kit - Gencyst
SKU: ELK1925
Availability: InStock

Human PON1-Paraoxonase 1 ELISA Kit

€318.00 – €455.00

Specification 96T, 48T

SKU: ELK1925 Category: ELISA Kit Tag: ELISA Kit

Overview of Human PON1-Paraoxonase 1 ELISA Kit

Product name:	Human PON1-Paraoxonase 1 ELISA Kit
Reactivity:	Human
Alternative Names:	ESA; PON; Esterase A; Serum paraoxonase/arylesterase 1; Aromatic esterase 1; A-esterase 1; Serum aryldialkylphosphatase 1
Assay Type:	Sandwich
Sensitivity:	1.27 ng/mL
Standard:	200 pg/mL
Detection Range:	3.13-200 ng/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5 h
Research Area:	Metabolic pathway;Endocrinology;
Test Principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PON1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PON1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PON1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PON1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Size	48T, 96T, 96T,48T

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