Chicken IL4-Interleukin 4 ELISA Kit

Chicken IL4-Interleukin 4 ELISA Kit

318.00455.00

Specification 96T, 48T

SKU: ELK2404 Category: Tag:

Overview of Chicken IL4-Interleukin 4 ELISA Kit

Product name: Chicken IL4-Interleukin 4 ELISA Kit
Reactivity: Chicken
Alternative Names: IL-4;BSF1; BCGF1 ; B Cell Stimulatory Factor 1; Lymphocyte stimulatory factor 1; B Cell Growth Factor; Binetrakin; Pitrakinra
Assay Type: Sandwich
Sensitivity: 6.3 pg/mL
Standard: 1000 ng/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5 h
Research Area: Cytokine;Immune molecule;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken IL4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken IL4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken IL4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken IL4 in the samples is then determined by comparing the OD of the samples to the standard curve.

Read More Description

Additional information

Size

48T, 96T, 96T,48T

Reviews

There are no reviews yet.

Be the first to review “Chicken IL4-Interleukin 4 ELISA Kit”

Your email address will not be published. Required fields are marked *

Filter by price