Name: General E2-Estradiol ELISA Kit - Gencyst
SKU: ELK1208
Availability: InStock

General E2-Estradiol ELISA Kit

€262.00 – €374.00

Specification 96T, 48T

SKU: ELK1208 Category: ELISA Kit Tag: ELISA Kit

Overview of E2-Estradiol ELISA Kit

Product name:	E2-Estradiol ELISA Kit
Reactivity:	General
Alternative Names:	17B-Estradiol; Oestradiol; Beta-Estradiol
Assay Type:	Competitive Inhibition
Sensitivity:	4.45 pg/mL
Standard:	1000 ng/mL
Detection Range:	15.63-1000 pg/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	2 h
Research Area:	Endocrinology;Reproductive science;Hormone metabolism;
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with E2 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to E2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of E2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Size	48T, 96T

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