General Testo-Testosterone ELISA Kit

General Testo-Testosterone ELISA Kit

262.00364.00

Specification 96T, 48T

SKU: ELK1332 Category: Tag:

Overview of Testo-Testosterone ELISA Kit

Product name: Testo-Testosterone ELISA Kit
Reactivity: General
Alternative Names: T; Testesteron; Testeronun
Assay Type: Competitive Inhibition
Sensitivity: 17.7 pg/mL
Standard: 4000 ng/mL
Detection Range: 62.5-4000 pg/mL
Sample Type: serum, plasma, other biological fluids
Assay Length: 2 h
Research Area: Endocrinology;Reproductive science;Hormone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Testo protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Testo. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Testo in the samples is then determined by comparing the OD of the samples to the standard curve.

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Additional information

Size

48T, 96T

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