Overview of Testo-Testosterone ELISA Kit
| Product name: | 
Testo-Testosterone ELISA Kit | 
| Reactivity: | 
General | 
| Alternative Names: | 
T; Testesteron; Testeronun | 
| Assay Type: | 
Competitive Inhibition | 
| Sensitivity: | 
17.7 pg/mL | 
| Standard: | 
4000 ng/mL | 
| Detection Range: | 
62.5-4000 pg/mL | 
| Sample Type: | 
serum, plasma, other biological fluids | 
| Assay Length: | 
2 h | 
| Research Area: | 
Endocrinology;Reproductive science;Hormone metabolism; | 
| Test principle: | 
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Testo protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Testo. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Testo in the samples is then determined by comparing the OD of the samples to the standard curve. | 
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