Human ALB-Albumin ELISA Kit

Human ALB-Albumin ELISA Kit

262.00364.00

Specification 96T, 48T

SKU: ELK1071 Category: Tag:

Overview of Human ALB-Albumin ELISA Kit

Product name: Human ALB-Albumin ELISA Kit
Reactivity: Human
Alternative Names: Serum albumin
Assay Type: Competitive Inhibition
Sensitivity: 89.6 ng/mL
Standard: 20000 pg/mL
Detection Range: 312.5-20000 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 2 h
Research Area: Tumor immunity;Infection immunity;Hematology;Hepatology;Nutrition metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human ALB protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ALB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ALB in the samples is then determined by comparing the OD of the samples to the standard curve.

Read More Description

dNTP Mix PCR Grade 10 mM each

Pfu DNA Polymerase

One Step SYBR Green RT-PCR Mix

Hot start Taq DNA Polymerase

EntiLink PCR Master Mix

Additional information

Size

48T, 96T, 96T,48T

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