Overview of Human AP12-Apelin 12 ELISA Kit
| Product name: | 
Human AP12-Apelin 12 ELISA Kit | 
| Reactivity: | 
Human | 
| Alternative Names: | 
APLN12 | 
| Assay Type: | 
Competitive Inhibition | 
| Sensitivity: | 
26.52 pg/mL | 
| Standard: | 
6000 ng/mL | 
| Detection Range: | 
93.75-6000 pg/mL | 
| Sample Type: | 
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids | 
| Assay Length: | 
2 h | 
| Research Area: | 
Metabolic pathway;Endocrinology;Cardiovascular biology;Gastroenterology;Hormone metabolism; | 
 | 
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human AP12 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AP12. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AP12 in the samples is then determined by comparing the OD of the samples to the standard curve. | 
dNTP Mix PCR Grade 10 mM each
Pfu DNA Polymerase
One Step SYBR Green RT-PCR Mix
Hot start Taq DNA Polymerase
EntiLink PCR Master Mix
					 
				
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