Human b2M(Beta-2-Microglobulin) ELISA Kit

Human b2M(Beta-2-Microglobulin) ELISA Kit


Specification 96T, 48T

SKU: ELK1200 Category: Tag:

Overview of Human BMG/b2-MG(Beta-2-Microglobulin) ELISA Kit

Product name: Human BMG/b2-MG(Beta-2-Microglobulin) ELISA Kit
Reactivity: Human
Alternative Names: Beta 2M; BMG; B2MG; b2M; Beta-2-Microglobulin
Assay Type: Sandwich
Sensitivity: 0.16 ng/mL
Standard: 25 ng/mL
Detection Range: 0.4-25 ng/mL
Sample Type: serum, plasma, urine and other biological fluids
Assay Length: 3.5h
Research Area: Infection immunity;Kidney biomarker;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BMG/b2-MG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BMG/b2-MG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human BMG/b2-MG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human BMG/b2-MG in the samples is then determined by comparing the OD of the samples to the standard curve.

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48T, 96T, 96T,48T

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