Overview of Human BMG/b2-MG-Beta-2-Microglobulin ELISA Kit
| Product name: |
Human BMG/b2-MG-Beta-2-Microglobulin ELISA Kit |
| Reactivity: |
Human |
| Alternative Names: |
Beta 2M; BMG; B2MG; b2M; Beta-2-Microglobulin |
| Assay Type: |
Sandwich |
| Sensitivity: |
0.16 ng/mL |
| Standard: |
25 ng/mL |
| Detection Range: |
0.4-25 ng/mL |
| Sample Type: |
serum, plasma, urine and other biological fluids |
| Assay Length: |
3.5 h |
| Research Area: |
Infection immunity;Kidney biomarker; |
| Test principle: |
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BMG/b2-MG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BMG/b2-MG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human BMG/b2-MG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human BMG/b2-MG in the samples is then determined by comparing the OD of the samples to the standard curve. |
dNTP Mix PCR Grade 10 mM each
Pfu DNA Polymerase
One Step SYBR Green RT-PCR Mix
Hot start Taq DNA Polymerase
EntiLink PCR Master Mix
