Human C1q(Complement 1q) ELISA Kit

Human C1q(Complement 1q) ELISA Kit

262.00364.00

Specification 96T, 48T

SKU: ELK1075 Category: Tag:

Overview of Human C1q(Complement 1q) ELISA Kit

Product name: Human C1q(Complement 1q) ELISA Kit
Reactivity: Human
Alternative Names: C1-q
Assay Type: Sandwich
Sensitivity: 0.137 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Infection immunity;Immune molecule;Autoimmunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human C1q. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human C1q. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human C1q, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human C1q in the samples is then determined by comparing the OD of the samples to the standard curve.

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Size

48T, 96T, 96T,48T