Human CYFRA21-1-Cytokeratin Fragment Antigen 21-1 ELISA Kit
Human CYFRA21-1-Cytokeratin Fragment Antigen 21-1 ELISA Kit
€318.00 – €455.00
Specification 96T, 48T
Overview of Human CYFRA21-1-Cytokeratin Fragment Antigen 21-1 ELISA Kit
Product name: |
Human CYFRA21-1-Cytokeratin Fragment Antigen 21-1 ELISA Kit |
Reactivity: | Human |
Alternative Names: | CYFRA21-1 |
Assay Type: | Sandwich |
Sensitivity: | 0.117 ng/mL |
Standard: | 20 ng/mL |
Detection Range: | 0.32-20 ng/mL |
Sample Type: | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
Assay Length: | 3.5 h |
Research Area: | Tumor immunity;Dermatology; |
Test Principle: | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CYFRA21-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CYFRA21-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CYFRA21-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CYFRA21-1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Additional information
Size | 48T, 96T |
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