Human FSH(Follicle Stimulating Hormone) ELISA Kit

Human FSH(Follicle Stimulating Hormone) ELISA Kit

364.00

Specification 96T, 48T

SKU: ELK1221 Category: Tag:

Overview of Human FSH(Follicle Stimulating Hormone) ELISA Kit

Product name: Human FSH(Follicle Stimulating Hormone) ELISA Kit
Reactivity: Human
Alternative Names: FSH-B; FSH-beta
Assay Type: Sandwich
Sensitivity: 0.34 mIU/mL
Standard: 50 mIU/mL
Detection Range: 0.79-50 mIU/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Endocrinology;Reproductive science;Genetic science;Hormone metabolism;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FSH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FSH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FSH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FSH in the samples is then determined by comparing the OD of the samples to the standard curve.

 

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Size

48T, 96T, 96T,48T

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