Human HB-Hemoglobin ELISA Kit
Human HB-Hemoglobin ELISA Kit
€262.00 – €364.00
Specification 96T, 48T
Overview of Human HB-Hemoglobin ELISA Kit
| Product name: | Human HB-Hemoglobin ELISA Kit |
| Reactivity: | Human |
| Alternative Names: | Hgb; Haemoglobin; Heterotetramer(αβ)2 |
| Assay Type: | Competitive Inhibition |
| Sensitivity: | 2.62 ÎĽg/mL |
| Standard: | 500 pg/mL |
| Detection Range: | 7.82-500 ÎĽg/mL |
| Sample Type: | serum, plasma, tissue homogenates, erythrocyte lysates, cell culture supernates and other biological fluids |
| Assay Length: | 2 h |
| Research Area: | Metabolic pathway;Infection immunity;Hematology;Neuro science; |
| Test principle: | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human APOA1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human APOA1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human APOA1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human APOA1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
Read More Description
Additional information
| Size | 48T, 96T, 96T,48T |
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