Name: Human HBF-Fetal Hemoglobin ELISA Kit - Gencyst
SKU: ELK1964
Availability: InStock

Human HBF-Fetal Hemoglobin ELISA Kit

€409.00 – €583.00

Specification 96T, 48T

SKU: ELK1964 Category: ELISA Kit Tag: ELISA Kit

Overview of Human HBF-Fetal Hemoglobin ELISA Kit

Product name:	Human HBF-Fetal Hemoglobin ELISA Kit
Reactivity:	Human
Alternative Names:	hemoglobin F; Foetal Haemoglobin
Assay Type:	Competitive Inhibition
Sensitivity:	0.99 ng/mL
Standard:	200 pg/mL
Detection Range:	3.13-200 ng/mL
Sample Type:	serum, plasma, erythrocyte lysates and other biological fluids
Assay Length:	2 h
Research Area:	Hematology;
Test Principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human HBF protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HBF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HBF in the samples is then determined by comparing the OD of the samples to the standard curve.

Size	48T, 96T

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