Human NT-ProANP-N-Terminal Pro-Atrial Natriuretic Peptide ELISA Kit
Human NT-ProANP-N-Terminal Pro-Atrial Natriuretic Peptide ELISA Kit
€409.00 – €583.00
Specification 96T, 48T
Overview of Human NT-ProANP-N-Terminal Pro-Atrial Natriuretic Peptide ELISA Kit
| Product name: | Human NT-ProANP-N-Terminal Pro-Atrial Natriuretic Peptide ELISA Kit |
| Reactivity: | Human |
| Alternative Names: | NT-Pro-ANP; N-ANP |
| Assay Type: | Sandwich |
| Sensitivity: | 14 pg/mL |
| Standard: | 2500 pg/mL |
| Detection Range: | 39.07-2500 pg/mL |
| Sample Type: | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay Length: | 3.5 h |
| Research Area: | Endocrinology;Cardiovascular biology; |
| Test Principle: | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NT-ProANP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NT-ProANP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NT-ProANP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NT-ProANP in the samples is then determined by comparing the OD of the samples to the standard curve. |
Read More Description
Additional information
| Size | 48T, 96T |
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