Name: Human PLGF-Placenta Growth Factor ELISA Kit - Gencyst
SKU: ELK1178
Availability: InStock

Human PLGF-Placenta Growth Factor ELISA Kit

€262.00 – €374.00

Specification 96T, 48T

Size

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SKU: ELK1178 Category: ELISA Kit Tag: ELISA Kit

Overview of Human PLGF-Placenta Growth Factor ELISA Kit

Product name:	Human PLGF-Placenta Growth Factor ELISA Kit
Reactivity:	Human
Alternative Names:	PlGF2; PGF; PGFL; Placental Growth Factor-Like; Vascular Endothelial Growth Factor-Related Protein
Assay Type:	Sandwich
Sensitivity:	6.5 pg/mL
Standard:	1000 pg/mL
Detection Range:	15.63-1000 pg/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5 h
Research Area:	Cytokine;Reproductive science;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PLGF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PLGF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PLGF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PLGF in the samples is then determined by comparing the OD of the samples to the standard curve.